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A Comprehensive Evaluation of PCR Primers to Amplify the nifH Gene of Nitrogenase
The nifH gene is the most widely sequenced marker gene used to identify nitrogen-fixing Bacteria and Archaea. Numerous PCR primers have been designed to amplify nifH, but a comprehensive evaluation of nifH PCR primers has not been performed. We performed an in silico analysis of the specificity and...
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| Main Authors: | , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
Public Library of Science
2012
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3405036/ https://ncbi.nlm.nih.gov/pubmed/22848735 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0042149 |
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