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Use of fluorescent protein to analyse recombination at three loci in Neurospora crassa

We have inserted a histone H1-GFP fusion gene adjacent to three loci on different chromosomes of Neurospora crassa and made mating pairs in which a wild type version of GFP is crossed to one with a mutation in the 5′ end of GFP. The loci are his-3, am and his-5, chosen because recombination mechanis...

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Detalhes bibliográficos
Main Authors: Bowring, F. J., Yeadon, P. J., Catcheside, D. E. A.
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3402616/
https://ncbi.nlm.nih.gov/pubmed/22691725
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.fgb.2012.05.012
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