Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms

DNA sequencing continues to decrease in cost with the Illumina HiSeq2000 generating up to 600 Gb of paired-end 100 base reads in a ten-day run. Here we present a protocol for community amplicon sequencing on the HiSeq2000 and MiSeq Illumina platforms, and apply that protocol to sequence 24 microbial...

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Bibliographische Detailangaben
Hauptverfasser: Caporaso, J Gregory, Lauber, Christian L, Walters, William A, Berg-Lyons, Donna, Huntley, James, Fierer, Noah, Owens, Sarah M, Betley, Jason, Fraser, Louise, Bauer, Markus, Gormley, Niall, Gilbert, Jack A, Smith, Geoff, Knight, Rob
Format: Artigo
Sprache:Inglês
Veröffentlicht: Nature Publishing Group 2012
Schlagworte:
Short Communication
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3400413/
https://ncbi.nlm.nih.gov/pubmed/22402401
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ismej.2012.8
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