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Functional Cloning and Expression of the Schizophyllum commune Glucuronoyl Esterase Gene and Characterization of the Recombinant Enzyme

The gene encoding Schizophyllum commune glucuronoyl esterase was identified in the scaffold 17 of the genome, containing two introns of 50 bp and 48 bp, with a transcript sequence of 1179 bp. The gene was synthesized and cloned into Pichia pastoris expression vector pGAPZα to achieve constitutive ex...

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Detalhes bibliográficos
Main Authors: Wong, Dominic W. S., Chan, Victor J., McCormack, Amanda A., Hirsch, Ján, Biely, Peter
Formato: Artigo
Idioma:Inglês
Publicado em: Hindawi Publishing Corporation 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3398583/
https://ncbi.nlm.nih.gov/pubmed/22844600
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2012/951267
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