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Sequences of three promoters for the bacteriophage SP6 RNA polymerase.
Fragments of SP6 DNA generated by cleavage with Hpa II or Taq I were cloned into the Cla I site of pBR322 and the recombinant plasmids were screened for the presence of SP6 promoter activity by transcription in vitro with purified SP6 RNA polymerase. Three plasmids having promoter activity and small...
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| Autors principals: | , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
1986
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| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC339790/ https://ncbi.nlm.nih.gov/pubmed/3010240 |
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