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The MURFI linker for multiple reading frame insertion of a sense or nonsense codon into DNA.
Blunt-end palindromic DNA linkers with a central restriction site have been designed for the multiple reading frame insertion (abbreviated MURFI) of a sense or nonsense codon into DNA. We have utilized an amber MURFI linker, 5'CTAG TCTAGA CTAG3' to disrupt the lacZ gene, yielding truncated...
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| Main Authors: | , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
1986
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| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC339648/ https://ncbi.nlm.nih.gov/pubmed/3008088 |
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