Stabilization of T4 polynucleotide kinase by macromolecular crowding.
T4 polynucleotide kinase rapidly loses activity during its reaction on duplex DNA termini. Addition of high concentrations of nonspecific polymers reverses or prevents this inactivation. In contrast, additions of related materials of lower molecular weight are relatively ineffective in stabilizing t...
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| Gepubliceerd in: | Nucleic Acids Res |
|---|---|
| Hoofdauteurs: | , |
| Formaat: | Artigo |
| Taal: | Inglês |
| Gepubliceerd in: |
Oxford University Press
1986
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| Online toegang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC339578/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3005982/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/14.4.1863 |
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