Testing the Limits of 454 Pyrotag Sequencing: Reproducibility, Quantitative Assessment and Comparison to T-RFLP Fingerprinting of Aquifer Microbes

The characterization of microbial community structure via 16S rRNA gene profiling has been greatly advanced in recent years by the introduction of amplicon pyrosequencing. The possibility of barcoding gives the opportunity to massively screen multiple samples from environmental or clinical sources f...

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Bibliographic Details
Main Authors: Pilloni, Giovanni, Granitsiotis, Michael S., Engel, Marion, Lueders, Tillmann
Format: Artigo
Language:Inglês
Published: Public Library of Science 2012
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3395703/
https://ncbi.nlm.nih.gov/pubmed/22808168
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0040467
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