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Eosinophil contamination of thioglycollate-elicited peritoneal macrophage cultures skews the functional readouts of in vitro assays

Thioglycollate-elicited peritoneal cells are a common source of macrophages for various in vitro assays, including stimulation with TLR ligands, cell signaling assays, phagocytosis, toxicology studies, and cytokine/chemokine production. The most common method for enrichment of cultured thioglycollat...

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Hlavní autoři: Misharin, Alexander V., Saber, Rana, Perlman, Harris
Médium: Artigo
Jazyk:Inglês
Vydáno: Society for Leukocyte Biology 2012
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3395415/
https://ncbi.nlm.nih.gov/pubmed/22706315
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1189/jlb.1111560
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