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A sensitive and rapid gel retention assay for nuclear factor I and other DNA-binding proteins in crude nuclear extracts.

The paper describes a rapid and sensitive assay for DNA binding proteins which interact with specific and defined binding sites. It exploits the observation that complexes of proteins and small synthetic DNA fragments (40 bp) containing the protein/DNA binding site can enter native polyacrylamide ge...

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Bibliografiske detaljer
Main Authors: Schneider, R, Gander, I, Müller, U, Mertz, R, Winnacker, E L
Format: Artigo
Sprog:Inglês
Udgivet: 1986
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC339505/
https://ncbi.nlm.nih.gov/pubmed/3513122
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