Error-Prone PCR Mutagenesis Reveals Functional Domains of a Bacterial Transcriptional Activator, TraJ

TraJ is the essential activator of P(Y), the promoter of the F and F-like plasmid tra operon that encodes the majority of the proteins for bacterial conjugation. By combining error-prone PCR mutagenesis with a two-plasmid screen, we isolated 55 missense mutations in traJ, each affecting the ability...

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Huvudupphovsmän: Lu, Jun, Peng, Yun, Arutyunov, Denis, Frost, Laura S., Glover, J. N. Mark
Materialtyp: Artigo
Språk:Inglês
Publicerad: American Society for Microbiology 2012
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Articles
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC3393494/
https://ncbi.nlm.nih.gov/pubmed/22563049
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.00312-12
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