A simple, economical and reproducible protein extraction protocol for proteomics studies of soybean roots

Sample preparation is a critical step in two-dimensional gel electrophoresis (2-DE) of plant tissues. Here we describe a phenol/SDS procedure that, although greatly simplified, produced well-resolved and reproducible 2-DE profiles of protein extracts from soybean [Glycine max (L.) Merril] roots. Ext...

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Detalhes bibliográficos
Main Authors: Rodrigues, Elisete Pains, Torres, Adalgisa Ribeiro, da Silva Batista, Jesiane Stefânia, Huergo, Luciano, Hungria, Mariangela
Formato: Artigo
Idioma:Inglês
Publicado em: Sociedade Brasileira de Genética 2012
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Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3392887/
https://ncbi.nlm.nih.gov/pubmed/22802720
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1590/S1415-47572012000200016
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