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Cloning and sequencing of the light chain variable region from NS-1 myeloma

The aim of this study was to clone the gene encoding the light chain variable region (V(L)) of the murine myeloma cell line P3/NS1/1-Ag4-1 (NS-1). Total RNA was prepared from the NS-1 cell line and its fusion hybridoma cell line 2F9. The V(L) gene was amplified using reverse transcription PCR (RT-PC...

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Bibliografiske detaljer
Main Authors: NING, BOTAO, TANG, YONGMIN, SONG, HUA, YANG, SHILONG, SHEN, HONGQIANG
Format: Artigo
Sprog:Inglês
Udgivet: D.A. Spandidos 2012
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3389680/
https://ncbi.nlm.nih.gov/pubmed/22783396
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3892/ol.2012.601
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