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Specialized binary vector for plant transformation: expression of the Arabidopsis thaliana AHAS gene in Nicotiana tabacum.

We constructed a cosmid vector, pOCA18, designed for transferring plant genomic libraries from Agrobacterium tumefaciens to plants. Clones from a genomic library of Arabidopsis thaliana DNA in pOCA 18 were propagated stably in both Escherichia coli and A. tumefaciens. Clones from the pOCA18 A. thali...

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Bibliographic Details
Main Authors: Olszewski, N E, Martin, F B, Ausubel, F M
Format: Artigo
Language:Inglês
Published: 1988
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC338938/
https://ncbi.nlm.nih.gov/pubmed/3060849
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