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Purification and crystallization of yeast Ent1 ENTH domain

Members of the Epsin protein family regulate the ubiquitin/clathrin-dependent trafficking of transmembrane proteins. The yeast Epsin-1 (ent1) gene was cloned and expressed in Escherichia coli. The protein product of a construct containing the ENTH-UIM modules was purified to homogeneity and subjecte...

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Detalhes bibliográficos
Main Authors: Tanner, Neta, Prag, Gali
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3388931/
https://ncbi.nlm.nih.gov/pubmed/22750874
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309112022488
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