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Crystallization and preliminary X-ray diffraction studies of the GhKCH2 motor domain: alteration of pH significantly improved the quality of the crystals

GhKCH2, a member of the kinesin superfamily, is a plant-specific microtubule-dependent motor protein from cotton with the ability to bind to both microtubules and microfilaments. Here, the motor domain of GhKCH2 (GhKCH2MD; amino acids 371–748) was overexpressed in Escherichia coli, purified and crys...

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Autors principals: Qin, Xinghua, Chen, Ziwei, Xu, Tao, Li, Ping, Liu, Guoqin
Format: Artigo
Idioma:Inglês
Publicat: International Union of Crystallography 2012
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3388925/
https://ncbi.nlm.nih.gov/pubmed/22750868
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309112016351
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