A microplate technique to simultaneously assay calcium accumulation in endoplasmic reticulum and SERCA release of inorganic phosphate

Traditional analyses of calcium homeostasis have separately quantified either calcium accumulation or release mechanisms. To define the system as a whole, however, requires multiple experimental techniques to examine both accumulation and release. Here we describe a technique that couples the simult...

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Autors principals: McMullen, David C, Kean, William S, Verma, Ajay, Cole, Jeffrey T, Watson, William D
Format: Artigo
Idioma:Inglês
Publicat: BioMed Central 2012
Matèries:
Methodology
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3388579/
https://ncbi.nlm.nih.gov/pubmed/22472432
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1480-9222-14-4
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