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Deletion of the Pichia pastoris KU70 Homologue Facilitates Platform Strain Generation for Gene Expression and Synthetic Biology

Targeted gene replacement to generate knock-outs and knock-ins is a commonly used method to study the function of unknown genes. In the methylotrophic yeast Pichia pastoris, the importance of specific gene targeting has increased since the genome sequencing projects of the most commonly used strains...

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Bibliographische Detailangaben
Hauptverfasser: Näätsaari, Laura, Mistlberger, Beate, Ruth, Claudia, Hajek, Tanja, Hartner, Franz S., Glieder, Anton
Format: Artigo
Sprache:Inglês
Veröffentlicht: Public Library of Science 2012
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3387205/
https://ncbi.nlm.nih.gov/pubmed/22768112
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0039720
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