DNA capture-probe based separation of double-stranded polymerase chain reaction amplification products in poly(dimethylsiloxane) microfluidic channels

Herein, we describe the development of a novel primer system that allows for the capture of double-stranded polymerase chain reaction (PCR) amplification products onto a microfluidic channel without any preliminary purification stages. We show that specially designed PCR primers consisting of the ma...

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Bibliographische Detailangaben
Hauptverfasser: Khodakov, Dmitriy, Thredgold, Leigh, Lenehan, Claire E., Andersson, Gunther G., Kobus, Hilton, Ellis, Amanda V.
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Institute of Physics 2012
Schlagworte:
Fabrication and Laboratory Methods
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3386992/
https://ncbi.nlm.nih.gov/pubmed/23761843
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1063/1.4729131
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