Characterization of the 1(st )and 2(nd )EF-hands of NADPH oxidase 5 by fluorescence, isothermal titration calorimetry, and circular dichroism

BACKGROUND: Superoxide generated by non-phagocytic NADPH oxidases (NOXs) is of growing importance for physiology and pathobiology. The calcium binding domain (CaBD) of NOX5 contains four EF-hands, each binding one calcium ion. To better understand the metal binding properties of the 1(st )and 2(nd )...

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Bibliographische Detailangaben
Hauptverfasser: Wei, Chin-Chuan, Reynolds, Nicole, Palka, Christina, Wetherell, Kristen, Boyle, Tiffany, Yang, Ya-Ping, Wang, Zhi-Qiang, Stuehr, Dennis J
Format: Artigo
Sprache:Inglês
Veröffentlicht: BioMed Central 2012
Schlagworte:
Research Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3386892/
https://ncbi.nlm.nih.gov/pubmed/22490336
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1752-153X-6-29
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