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Repeated integration of antibody genes into a pre-selected chromosomal locus of CHO cells using an accumulative site-specific gene integration system

We previously reported an accumulative site-specific gene integration system using Cre recombinase and mutated loxP sites, where a recombinase-mediated cassette exchange (RMCE) reaction is repeatable. This gene integration system was applied for antibody production using recombinant Chinese hamster...

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Detalhes bibliográficos
Main Authors: Kawabe, Yoshinori, Makitsubo, Hirokatsu, Kameyama, Yujiro, Huang, Shuohao, Ito, Akira, Kamihira, Masamichi
Formato: Artigo
Idioma:Inglês
Publicado em: Springer Netherlands 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3386388/
https://ncbi.nlm.nih.gov/pubmed/21948097
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10616-011-9397-y
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