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Nuclear export of single native mRNA molecules observed by light sheet fluorescence microscopy

Nuclear export of mRNA is a key transport process in eukaryotic cells. To investigate it, we labeled native mRNP particles in living Chironomus tentans salivary gland cells with fluorescent hrp36, the hnRNP A1 homolog, and the nuclear envelope by fluorescent NTF2. Using light sheet microscopy, we tr...

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Detalhes bibliográficos
Main Authors: Siebrasse, Jan Peter, Kaminski, Tim, Kubitscheck, Ulrich
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3386080/
https://ncbi.nlm.nih.gov/pubmed/22615357
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1201781109
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