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Artificial mirtron-mediated gene knockdown: Functional DMPK silencing in mammalian cells

Mirtrons are introns that form pre-miRNA hairpins after splicing to produce RNA interference (RNAi) effectors distinct from Drosha-dependent intronic miRNAs. Here we present a design algorithm for artificial mirtrons and demonstrate, for the first time, efficient gene knockdown of myotonic dystrophy...

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Hlavní autoři: Seow, Yiqi, Sibley, Christopher R., Wood, Matthew J.A.
Médium: Artigo
Jazyk:Inglês
Vydáno: Cold Spring Harbor Laboratory Press 2012
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3383964/
https://ncbi.nlm.nih.gov/pubmed/22647847
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.030601.111
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