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Purification and Recognition of Recombinant Mouse P2X(1) Receptors Expressed in a Baculovirus System

[Table: see text] The hexahistidine-tagged mouse P2X(1) receptor (H-mP2X(1)R), an ATP-gated ion channel receptor, was expressed in a baculovirus system using the pAcHLT-B transfer vector containing a hexahistidine tag. Both widely used denaturing (8M urea) and nondenaturing (such as 1% Triton X-100)...

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書誌詳細
主要な著者: Chen, Liping, Hardwick, James P., McPhie, Peter, Sitkovsky, Michail V., Jacobson, Kenneth A.
フォーマット: Artigo
言語:Inglês
出版事項: 2000
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC3383047/
https://ncbi.nlm.nih.gov/pubmed/22745520
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/1098-2299(20000901)51:1<7::AID-DDR2>3.0.CO;2-W
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