Rapid, large-scale purification and characterization of 'Ada protein' (O6 methylguanine-DNA methyltransferase) of E. coli.

The E. coli Ada protein (O6-methylguanine-DNA methyltransferase) has been purified using a high-level expression vector with a yield of about 3 mg per liter of E. coli culture. The 39-kDa protein has an extinction coefficient (E280 nm (1%)) of 5.3. Its isoelectric point of 7.1 is lower than that pre...

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Vydáno v:	Nucleic Acids Res
Hlavní autoři:	Bhattacharyya, D, Tano, K, Bunick, G J, Uberbacher, E C, Behnke, W D, Mitra, S
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Oxford University Press 1988
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC338304/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3041376/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/16.14.6397
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