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Methodological considerations for global analysis of cellular FLIM/FRET measurements
Global algorithms can improve the analysis of fluorescence energy transfer (FRET) measurement based on fluorescence lifetime microscopy. However, global analysis of FRET data is also susceptible to experimental artifacts. This work examines several common artifacts and suggests remedial experimental...
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| Главные авторы: | , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Society of Photo-Optical Instrumentation Engineers
2012
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3382354/ https://ncbi.nlm.nih.gov/pubmed/22463045 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1117/1.JBO.17.2.026013 |
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