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Real-time Measurement of Pyrophosphate Release Kinetics

In order to measure the kinetics of pyrophosphate release, we have coupled pyrophosphatase to the fluorescently labeled phosphate binding protein developed by Webb. We show that the assay is capable of measuring rates of pyrophosphate release up to 100 s(−1).

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Autori principali: Hanes, Jeremiah W., Johnson, Kenneth A.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2007
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3381508/
https://ncbi.nlm.nih.gov/pubmed/17905190
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2007.08.004
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