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Label-free separation of human embryonic stem cells and their differentiating progenies by phasor fluorescence lifetime microscopy
We develop a label-free optical technique to image and discriminate undifferentiated human embryonic stem cells (hESCs) from their differentiating progenies in vitro. Using intrinsic cellular fluorophores, we perform fluorescence lifetime microscopy (FLIM) and phasor analysis to obtain hESC metaboli...
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| Autores principales: | , , , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Society of Photo-Optical Instrumentation Engineers
2012
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3381030/ https://ncbi.nlm.nih.gov/pubmed/22559690 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1117/1.JBO.17.4.046012 |
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