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A multiphoton objective design with incorporated beam splitter for enhanced fluorescence collection

We present a de novo design of an objective for use in multi-photon (MPM) and second harmonic generation (SHG) microscopy. This objective was designed to have a large field of view (FOV), while maintaining a moderate numerical aperture (NA) and relative straight forward construction. A dichroic beam...

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Autors principals: McMullen, Jesse D., Zipfel, Warren R.
Format: Artigo
Idioma:Inglês
Publicat: Optical Society of America 2010
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3378349/
https://ncbi.nlm.nih.gov/pubmed/20389554
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/OE.18.005390
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