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Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli
The efficient generation of genetic diversity represents an invaluable molecular tool that can be used to label DNA synthesis, to create unique molecular signatures, or to evolve proteins in the laboratory. Here, we present a protocol that allows the generation of large (>10(11)) mutant libraries...
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| Auteurs principaux: | , , , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
MyJove Corporation
2011
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3376869/ https://ncbi.nlm.nih.gov/pubmed/21445044 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/2505 |
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