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The role of the oligosaccharide binding cleft of rice BGlu1 in hydrolysis of cellooligosaccharides and in their synthesis by rice BGlu1 glycosynthase

Rice BGlu1 β-glucosidase nucleophile mutant E386G is a glycosynthase that can synthesize p-nitrophenyl (pNP)-cellooligosaccharides of up to 11 residues. The X-ray crystal structures of the E386G glycosynthase with and without α-glucosyl fluoride were solved and the α-glucosyl fluoride complex was fo...

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Détails bibliographiques
Auteurs principaux: Pengthaisong, Salila, Withers, Stephen G, Kuaprasert, Buabarn, Svasti, Jisnuson, Ketudat Cairns, James R
Format: Artigo
Langue:Inglês
Publié: Wiley Subscription Services, Inc., A Wiley Company 2012
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3375437/
https://ncbi.nlm.nih.gov/pubmed/22238157
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.2021
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