Crystallization and preliminary crystallographic studies of both components of the staphylococcal LukE–LukD leukotoxin

Soluble forms of recombinant LukE protein (expressed in Escherichia coli) and of wild-type LukD protein (expressed in Staphylococcus aureus), which together form the staphylococcal LukE–LukD leukotoxin, were purified to homogeneity and crystallized using the sitting-drop vapour-diffusion method. The...

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Bibliographic Details
Main Authors: Galy, Romain, Bergeret, Fabien, Keller, Daniel, Mourey, Lionel, Prévost, Gilles, Maveyraud, Laurent
Format: Artigo
Language:Inglês
Published: International Union of Crystallography 2012
Subjects:
Crystallization Communications
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3370905/
https://ncbi.nlm.nih.gov/pubmed/22684065
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309112014662
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