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High-Throughput PCR Assays To Monitor Wolbachia Infection in the Dengue Mosquito (Aedes aegypti) and Drosophila simulans

We have developed and validated two new fluorescence-based PCR assays to detect the Wolbachia wMel strain in Aedes aegypti and the wRi and wAu strains in Drosophila simulans. The new assays are accurate, informative, and cost-efficient for large-scale Wolbachia screening.

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Detalhes bibliográficos
Main Authors: Lee, Siu F., White, Vanessa L., Weeks, Andrew R., Hoffmann, Ary A., Endersby, Nancy M.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3370494/
https://ncbi.nlm.nih.gov/pubmed/22522691
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00069-12
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