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Impact of wavefront distortion and scattering on 2-photon microscopy in mammalian brain tissue

Two-photon (2P) microscopy is widely used in neuroscience, but the optical properties of brain tissue are poorly understood. We have investigated the effect of brain tissue on the 2P point spread function (PSF(2P)) by imaging fluorescent beads through living cortical slices. By combining this with m...

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Detalhes bibliográficos
Main Authors: Chaigneau, Emmanuelle, Wright, Amanda J., Poland, Simon P., Girkin, John M., Silver, R. Angus
Formato: Artigo
Idioma:Inglês
Publicado em: Optical Society of America 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3369558/
https://ncbi.nlm.nih.gov/pubmed/22109156
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/OE.19.022755
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