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Site-directed mutagenesis in the DNA linking site of bacteriophage phi 29 terminal protein: isolation and characterization of a Ser232----Thr mutant.

By site-directed mutagenesis we have changed the serine residue 232 of the phi 29 terminal protein, involved in the covalent linkage to dAMP for the initiation of replication, into a threonine residue. The mutant terminal protein has been purified to homogeneity and shown to be inactive in the forma...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Garmendia, C, Salas, M, Hermoso, J M
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1988
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC336825/
https://ncbi.nlm.nih.gov/pubmed/3135531
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