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Determination of the molecular weight of DNA-bound protein(s) responsible for gel electrophoretic mobility shift of linear DNA fragments examplified with purified viral myb protein.
A protein-DNA complex has less gel electrophoretic mobility than the free DNA fragment. One parameter for the degree of retardation of a linear DNA fragment in a protein-DNA complex is the molecular weight of the bound protein(s). The quotient of the migration distances of free DNA (m) and protein-D...
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
1988
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| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC336764/ https://ncbi.nlm.nih.gov/pubmed/2968540 |
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