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In vitro transcription and translational efficiency of chimeric SP6 messenger RNAs devoid of 5' vector nucleotides.

A plasmid containing the bacteriophage SP6 promoter, designated pHSTO, permits in vitro transcription of RNAs devoid of vector-derived nucleotides. This vector has been characterized for relative transcriptional activity using constructs which alter the conserved nucleotides extending beyond the SP6...

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Detalhes bibliográficos
Main Authors: Jobling, S A, Cuthbert, C M, Rogers, S G, Fraley, R T, Gehrke, L
Formato: Artigo
Idioma:Inglês
Publicado em: 1988
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC336643/
https://ncbi.nlm.nih.gov/pubmed/3260027
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