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Exploiting Ligand-Protein Conjugates to Monitor Ligand-Receptor Interactions

We introduce three assays for analyzing ligand-receptor interactions based on the specific conjugation of ligands to SNAP-tag fusion proteins. Conjugation of ligands to different SNAP-tag fusions permits the validation of suspected interactions in cell extracts and fixed cells as well as the establi...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Haruki, Hirohito, Gonzalez, Monica Rengifo, Johnsson, Kai
Format: Artigo
Sprache:Inglês
Veröffentlicht: Public Library of Science 2012
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3365113/
https://ncbi.nlm.nih.gov/pubmed/22701522
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0037598
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