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DNA supercoiling by Xenopus laevis oocyte extracts: requirement for a nuclear factor.

A purified system is described for the introduction of negative supercoils into simian virus 40 DNA. The system consists of histones H2A, H2B, H3, and H4, DNA-relaxing enzyme, and a purified factor from Xenopus laevis stage 6 oocyte nuclei. The nuclei are prepared en masse by the technique of F. Sca...

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Veröffentlicht in:Proc Natl Acad Sci U S A
Hauptverfasser: Baldi, M I, Mattoccia, E, Tocchini-Valentini, G P
Format: Artigo
Sprache:Inglês
Veröffentlicht: National Academy of Sciences 1978
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Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC336223/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/217004/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.75.10.4873
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