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Metal Reconstitution of Particulate Methane Monooxygenase and Heterologous Expression of the pmoB Subunit
Particulate methane monooxygenase (pMMO) is a multisubunit metalloenzyme complex used by methanotrophic bacteria to oxidize methane in the first step of carbon assimilation and energy production. In this chapter, we detail methods to prepare metal free (apo) membrane-bound pMMO and to reconstitute a...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3361753/ https://ncbi.nlm.nih.gov/pubmed/21419923 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/B978-0-12-386905-0.00013-9 |
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