Energy-dependent binding of dansylgalactoside to the lac carrier protein: direct binding measurements.

High specific activity 6'-N-[3H]dansyl)aminohexyl 1-thio-beta-D-galactopyranoside (Dns6-Gal) has been synthesized, and its binding to Escherichia coli membrane vesicles measured directly by flow dialysis. With ML 308-225 vesicles containing the lac carrier protein, specific binding is not detec...

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Bibliographic Details
Main Authors: Schuldiner, S, Weil, R, Kaback, H R
Format: Artigo
Language:Inglês
Published: 1976
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC335849/
https://ncbi.nlm.nih.gov/pubmed/174094
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