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IMMUNOGLOBULIN SYNTHESIS AND SECRETION, V. INCORPORATION OF LEUCINE AND GLUCOSAMINE INTO IMMUNOGLOBULIN ON FREE AND BOUND POLYRIBOSOMES

Mouse myeloma cells pulse-labeled in vitro with (3)H-leucine or (3)H-glucosamine were fractionated on sucrose gradients, and membrane-bound and free polyribosomes were separated. Nascent polypeptides released from polyribosomes were precipitated with antiserum specific for mouse immunoglobulin. The...

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Bibliografiske detaljer
Main Authors: Sherr, Charles J., Uhr, Jonathan W.
Format: Artigo
Sprog:Inglês
Udgivet: 1970
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC335804/
https://ncbi.nlm.nih.gov/pubmed/5273448
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