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Hyperexpression and purification of Escherichia coli adenylate cyclase using a vector designed for expression of lethal gene products.
We describe the construction of a new generation of vectors (pRE) for the hyperexpression of lethal gene products such as adenylate cyclase in Escherichia coli. The pRE vectors are based on the lambda PL promoter and lambda cII ribosome binding site described by Shimatake and Rosenberg (Nature, 292,...
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| Publicado no: | Nucleic Acids Res |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
1989
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| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC335313/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2557591/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/17.24.10473 |
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