Non-Bias-Limited Tracking of Spherical Particles, Enabling Nanometer Resolution at Low Magnification

We present a three-dimensional tracking routine for nondiffraction-limited particles, which significantly reduces pixel bias. Our technique allows for increased resolution compared to that of previous methods, especially at low magnification or at high signal/noise ratio. This enables tracking with...

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Dades bibliogràfiques
Autors principals: van Loenhout, Marijn T.J., Kerssemakers, Jacob W.J., De Vlaminck, Iwijn, Dekker, Cees
Format: Artigo
Idioma:Inglês
Publicat: The Biophysical Society 2012
Matèries:
Spectroscopy, Imaging, and Other Techniques
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3353059/
https://ncbi.nlm.nih.gov/pubmed/22677390
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2012.03.073
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