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High-throughput Method Development for Sensitive, Accurate and Reproducible Quantification of Therapeutic Monoclonal Antibodies in Tissues Using Orthogonal Array Optimization and Nano-LC/SRM-MS

Although liquid chromatography/mass spectrometry using selected reaction monitoring (LC/SRM-MS) holds great promise for targeted protein analysis, quantification of therapeutic monoclonal antibody (mAb) in tissues represents a daunting challenge due to the extremely-low tissue levels, complexity of...

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Main Authors: Duan, Xiaotao, Abuqayyas, Lubna, Dai, Lipeng, Balthasar, Joseph P., Qu, Jun
Format: Artigo
Jezik:Inglês
Izdano: 2012
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3352998/
https://ncbi.nlm.nih.gov/pubmed/22519810
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac2034166
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