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Fusion of a fluorescent protein to the pUL25 minor capsid protein of pseudorabies virus allows live-cell capsid imaging with negligible impact on infection

In order to resolve the location and activity of submicroscopic viruses in living cells, viral proteins are often fused to fluorescent proteins (FPs) and visualized by microscopy. In this study, we describe the fusion of FPs to three proteins of pseudorabies virus (PRV) that allowed imaging of capsi...

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Autors principals: Bohannon, Kevin P., Sollars, Patricia J., Pickard, Gary E., Smith, Gregory A.
Format: Artigo
Idioma:Inglês
Publicat: Society for General Microbiology 2012
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3352333/
https://ncbi.nlm.nih.gov/pubmed/21976610
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1099/vir.0.036145-0
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