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The presence of the region on pBR322 that encodes resistance to tetracycline is responsible for high levels of plasmid DNA knotting in Escherichia coli DNA topoisomerase I deletion mutant.

Plasmid pBR322 DNA isolated from Escherichia coli DNA topoisomerase I deletion mutant DM800 is estimated to contain about 10% of the knotted forms (Shishido et al., 1987). These knotted DNA species were shown to have the same primary structure as usual, unknotted pBR322 DNA. Analysis of the knotting...

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Sonraí Bibleagrafaíochta
Main Authors: Shishido, K, Ishii, S, Komiyama, N
Formáid: Artigo
Teanga:Inglês
Foilsithe: 1989
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC335211/
https://ncbi.nlm.nih.gov/pubmed/2557587
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