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The use of 5'-phospho-2 deoxyribocytidylylriboadenosine as a facile route to chemical aminoacylation of tRNA.

Methodology is described for the synthesis and chemical aminoacylation of the hybrid dinucleotide 5'-phospho-2'-deoxyribocytidylylriboadenosine (pdCpA). Ligation of aminoacylated pdCpA to a truncated amber suppressor tRNACUA (-CA) using T4 RNA ligase generates an aminoacylated suppressor tRNA which...

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Udgivet i:Nucleic Acids Res
Principais autores: Robertson, S A, Noren, C J, Anthony-Cahill, S J, Griffith, M C, Schultz, P G
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1989
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC335204/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2602139/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/17.23.9649
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