Purification of the E. coli ogt gene product to homogeneity and its rate of action on O6-methylguanine, O6-ethylguanine and O4-methylthymine in dodecadeoxyribonucleotides.

The E. coli gene ogt encodes the DNA repair protein O6-alkylguanine-DNA-alkyltransferase (O6-AlkG ATase). The protein coding region of the gene was cloned into a multicopy expression vector to obtain high yields of the enzyme (approximately 0.2% of total protein) which was purified to apparent homog...

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Bibliografski detalji

Izdano u:	Nucleic Acids Res
Glavni autori:	Wilkinson, M C, Potter, P M, Cawkwell, L, Georgiadis, P, Patel, D, Swann, P F, Margison, G P
Format:	Artigo
Jezik:	Inglês
Izdano:	Oxford University Press 1989
Online pristup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC335020/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2685744/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/17.21.8475
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