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One-step generation of error-prone PCR libraries using Gateway(® )technology
BACKGROUND: Error-prone PCR (epPCR) libraries are one of the tools used in directed evolution. The Gateway(® )technology allows constructing epPCR libraries virtually devoid of any background (i.e., of insert-free plasmid), but requires two steps: the BP and the LR reactions and the associated E. co...
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| Main Authors: | , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
BioMed Central
2012
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3349575/ https://ncbi.nlm.nih.gov/pubmed/22289297 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1475-2859-11-14 |
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